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7/21/2019 01.INTRODUCERE.ppt
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METODELE DE STUDIUMETODELE DE STUDIU
UTILIZATE ÎNUTILIZATE ÎNMORFOPATOLOGIEMORFOPATOLOGIE
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P a t o l o g i a
Medicina clinică
Puntea de legătură
Ştiinţele fundamentale
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SubdiviSubdivizziiunileunile PatologPatologieiiei:: HistopatologHistopatologiaia CCiitopatologtopatologiaia HematologHematologiaia MicrobiologMicrobiologiaia ImunologImunologiaia Patologia chimicăPatologia chimică Patologia geneticăPatologia genetică ToxicologToxicologiaia
Medicina legalăMedicina legală
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OBIECTELE PATOLOGIEIOBIECTELE PATOLOGIEI
Materialul cadaveric.Materialul cadaveric. Ţeuturile ob!inute Ţeuturile ob!inute
intravital"co#$acte %i lic&ide'.intravital"co#$acte %i lic&ide'. Material e($eri#ental.Material e($eri#ental.
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Metodele de invetiga!ie )nMetodele de invetiga!ie )n$atologie.$atologie.
Metoda &itologic*Metoda &itologic* Metoda citologic*Metoda citologic* Metode i#uno&itoc&i#iceMetode i#uno&itoc&i#ice Metodele biologiei #oleculareMetodele biologiei #oleculare
Metodele geneticeMetodele genetice Microco$ia electronic*Microco$ia electronic* Metode i#agiticeMetode i#agitice
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Metode #icroco$ice utilizateMetode #icroco$ice utilizate
la #o#entul actualla #o#entul actual Microco$ia o$tic*Microco$ia o$tic* Microco$ia cu contrat de +az*Microco$ia cu contrat de +az* Microco$ia ,uorecent*Microco$ia ,uorecent* Microco$ia )n c)#$ )ntunecatMicroco$ia )n c)#$ )ntunecat
Microco$ia electronic*Microco$ia electronic*
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A-TOPSIAA-TOPSIA
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MATEIAL POSTOPEATOMATEIAL POSTOPEATO
APE/0ICELE 1EMIC-LA
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BIOPSIA LAPAOSCOPIC2BIOPSIA LAPAOSCOPIC2
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BIOPSIA PI/ E3CI4IEBIOPSIA PI/ E3CI4IE
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BIOPSIA E/0OSCOPIC2BIOPSIA E/0OSCOPIC2
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Bio$ia cu ac ub!ireBio$ia cu ac ub!ire
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5/A $rin -SG g&idare5/A $rin -SG g&idare
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Metoda e+ectu*rii 5/AMetoda e+ectu*rii 5/A
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Colectarea #aterialuluiColectarea #aterialului
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Materialul bioptic
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Bio$6 7 Slide $re$arationBio$6 7 Slide $re$aration
C tti f ti
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Cutting a frozen section.
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colorareacolorarea
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Acoperirea cu lamele
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Mi i ti *Mi i ti *
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Microco$ia o$tic*Microco$ia o$tic*
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8e#ato(ilin*9Eozin*8e#ato(ilin*9Eozin*
d
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o%u de Congoo%u de Congo
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Albatru de $ruiaAlbatru de $ruia
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Colora!ia Tric&ro#Colora!ia Tric&ro#
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IM-/O5L-OESCE/ŢAIM-/O5L-OESCE/ŢA
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/eutro$&il nor#al electron #icrogra$&.
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5LO;CITOMETIA5LO;CITOMETIA
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488 nm laser
+-
Fluorescence Activated
Cell Sorting
Charged Plates
Single cells sorted
into test tubes
FALS Sensor
Fluorescence detector
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CO0 /OMALCO0 /OMAL
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PATOLOGICPATOLOGIC